Identification and function of auxiliary iron-sulfur clusters in radical SAM enzymes.

Biochimica et biophysica acta 20121101

Radical SAM enzymes involved in the biosynthesis of purine-based natural products.

Biochimica et biophysica acta 20121101

Radical SAM enzymes in methylation and methylthiolation.

Metallomics : integrated biometal science 20121101

Hydrogen bonds guide the short-lived 5'-deoxyadenosyl radical to the place of action.

Angewandte Chemie (International ed. in English) 20121001

Radical SAM-dependent carbon insertion into the nitrogenase M-cluster.

Science (New York, N.Y.) 20120928

9-Mercaptodethiobiotin is generated as a ligand to the [2Fe-2S]+ cluster during the reaction catalyzed by biotin synthase from Escherichia coli.

Journal of the American Chemical Society 20120606

Radical-mediated enzymatic methylation: a tale of two SAMS.

Accounts of chemical research 20120417

Induction of intracellular calcium concentration by environmental benzo(a)pyrene involves a β2-adrenergic receptor/adenylyl cyclase/Epac-1/inositol 1,4,5-trisphosphate pathway in endothelial cells.

The Journal of biological chemistry 20120203

The elusive 5'-deoxyadenosyl radical in coenzyme-B12-mediated reactions.

Journal of the American Chemical Society 20120125

RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins.

Methods in enzymology 20120101

Cfr and RlmN contain a single [4Fe-4S] cluster, which directs two distinct reactivities for S-adenosylmethionine: methyl transfer by SN2 displacement and radical generation.

Journal of the American Chemical Society 20111214

S K-edge XAS and DFT calculations on SAM dependent pyruvate formate-lyase activating enzyme: nature of interaction between the Fe4S4 cluster and SAM and its role in reactivity.

Journal of the American Chemical Society 20111123

Pyridoxal-5'-phosphate as the catalyst for radical isomerization in reactions of PLP-dependent aminomutases.

Biochimica et biophysica acta 20111101

Reduction of the [2Fe-2S] cluster accompanies formation of the intermediate 9-mercaptodethiobiotin in Escherichia coli biotin synthase.

Biochemistry 20110920

Determination of cobalamins (hydroxo-, cyano-, adenosyl- and methyl-cobalamins) in seawater using reversed-phase liquid chromatography with diode-array detection.

Analytica chimica acta 20110902

Complex biotransformations catalyzed by radical S-adenosylmethionine enzymes.

The Journal of biological chemistry 20110902

Multiple pathways from three types of sugar receptor sites to metabotropic transduction pathways of the blowfly: study by the whole cell-clamp experiments.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 20110901

Crystal structure of PduO-Type ATP:Cob(I)alamin adenosyltransferase from Bacillus cereus in a complex with ATP.

Biochemical and biophysical research communications 20110513

Inhibitory effects of indicaxanthin on mouse ileal contractility: analysis of the mechanism of action.

European journal of pharmacology 20110511

A radically different mechanism for S-adenosylmethionine-dependent methyltransferases.

Science (New York, N.Y.) 20110429

Radicals from S-adenosylmethionine and their application to biosynthesis.

Current opinion in chemical biology 20110401

Role of prostanoid IP and EP receptors in mediating vasorelaxant responses to PGI2 analogues in rat tail artery: Evidence for Gi/o modulation via EP3 receptors.

European journal of pharmacology 20110311

Identification of 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) receptor binding and membrane progestin receptor alpha on southern flounder sperm (Paralichthys lethostigma) and their likely role in 20β-S stimulation of sperm hypermotility.

General and comparative endocrinology 20110201

Radical SAM activation of the B12-independent glycerol dehydratase results in formation of 5'-deoxy-5'-(methylthio)adenosine and not 5'-deoxyadenosine.

Biochemistry 20110201

Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis.

Molecular bioSystems 20110101

Time-resolved in silico modeling of fine-tuned cAMP signaling in platelets: feedback loops, titrated phosphorylations and pharmacological modulation.

BMC systems biology 20110101

High-level expression and reconstitution of active Cfr, a radical-SAM rRNA methyltransferase that confers resistance to ribosome-acting antibiotics.

Protein expression and purification 20101201

Structural basis for adenosylcobalamin activation in AdoCbl-dependent ribonucleotide reductases.

ACS chemical biology 20101015

Diphthamide biosynthesis requires an organic radical generated by an iron-sulphur enzyme.

Nature 20100617

A consensus mechanism for Radical SAM-dependent dehydrogenation? BtrN contains two [4Fe-4S] clusters.

Biochemistry 20100511

Large-scale domain dynamics and adenosylcobalamin reorientation orchestrate radical catalysis in ornithine 4,5-aminomutase.

The Journal of biological chemistry 20100430

Anaerobic sulfatase-maturating enzyme--a mechanistic link with glycyl radical-activating enzymes?

The FEBS journal 20100401

RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA.

Journal of the American Chemical Society 20100324

Vitamin B12 in health and disease.

Nutrients 20100301

Catalytic activity of the anaerobic tyrosine lyase required for thiamine biosynthesis in Escherichia coli.

The Journal of biological chemistry 20100219

Characterisation of PduS, the pdu metabolosome corrin reductase, and evidence of substructural organisation within the bacterial microcompartment.

PloS one 20100101

Mechanistic and functional versatility of radical SAM enzymes.

F1000 biology reports 20100101

Stimulation of the cAMP system by the nitric oxide-cGMP system underlying the formation of long-term memory in an insect.

Neuroscience letters 20091225

Ligand-binding by catalytically inactive mutants of the cblB complementation group defective in human ATP:cob(I)alamin adenosyltransferase.

Molecular genetics and metabolism 20091101

Pyrroloquinoline quinone biogenesis: demonstration that PqqE from Klebsiella pneumoniae is a radical S-adenosyl-L-methionine enzyme.

Biochemistry 20091027

Characterization and mechanistic studies of DesII: a radical S-adenosyl-L-methionine enzyme involved in the biosynthesis of TDP-D-desosamine.

Journal of the American Chemical Society 20091007

Glucagon-like peptide-1 protects mesenteric endothelium from injury during inflammation.

Peptides 20090901

Processing of alkylcobalamins in mammalian cells: A role for the MMACHC (cblC) gene product.

Molecular genetics and metabolism 20090801

Gastric relaxation induced by apigenin and quercetin: analysis of the mechanism of action.

Life sciences 20090703

Delineation of the chemomechanosensory regulation of gastrin secretion using pure rodent G cells.

Gastroenterology 20090701

Biochemical characterization of the GTP:adenosylcobinamide-phosphate guanylyltransferase (CobY) enzyme of the hyperthermophilic archaeon Methanocaldococcus jannaschii.

Biochemistry 20090630

Mechanistic investigations of anaerobic sulfatase-maturating enzyme: direct Cbeta H-atom abstraction catalyzed by a radical AdoMet enzyme.

Journal of the American Chemical Society 20090624

Stabilization of the base-off forms of vitamin B(12) and coenzyme B(12) by encapsulation of the alpha-axial 5,6-dimethylbenzimidazole ligand with cucurbit[7]uril.

Dalton transactions (Cambridge, England : 2003) 20090514

Product inhibition in the radical S-adenosylmethionine family.

FEBS letters 20090417

DFT and ONIOM(DFT:MM) studies on Co-C bond cleavage and hydrogen transfer in B12-dependent methylmalonyl-CoA mutase. Stepwise or concerted mechanism?

Journal of the American Chemical Society 20090415

Protein residues that control the reaction trajectory in S-adenosylmethionine radical enzymes: mutagenesis of asparagine 153 and aspartate 155 in Escherichia coli biotin synthase.

Biochemistry 20090324

Tramadol-induced block of hyperpolarization-activated cation current in rat pituitary lactotrophs.

Naunyn-Schmiedeberg's archives of pharmacology 20090201

Anaerobic functionalization of unactivated C-H bonds.

Current opinion in chemical biology 20090201

Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization.

BMC genomics 20090101

A comparative genomics study of genetic products potentially encoding ladderane lipid biosynthesis.

Biology direct 20090101

Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation.

PloS one 20090101

9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase.

Biochemistry 20080902

Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy.

Biochemistry 20080826

In vitro characterization of AtsB, a radical SAM formylglycine-generating enzyme that contains three [4Fe-4S] clusters.

Biochemistry 20080715

Crystallization and preliminary X-ray crystallographic studies of a PduO-type ATP:cob(I)alamin adenosyltransferase from Bacillus cereus.

Acta crystallographica. Section F, Structural biology and crystallization communications 20080701

Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate intermediate.

Biochemistry 20080527

Novel targets for Huntington's disease in an mTOR-independent autophagy pathway.

Nature chemical biology 20080501

Effect of cyclic AMP on barrier function of human lymphatic microvascular tubes.

Microvascular research 20080501

Lipolysis is stimulated by PEGylated conjugated linoleic acid through the cyclic adenosine monophosphate-independent signaling pathway in 3T3-L1 cells: activation of MEK/ERK MAPK signaling pathway and hyper-secretion of adipo-cytokines.

Journal of cellular physiology 20080201

Relaxant action mechanism of berberine identified as the active principle of Argemone ochroleuca Sweet in guinea-pig tracheal smooth muscle.

The Journal of pharmacy and pharmacology 20080201

The Radical SAM Superfamily.

Critical reviews in biochemistry and molecular biology 20080101

The pyrroloquinoline quinone biosynthesis pathway revisited: a structural approach.

BMC biochemistry 20080101

Adiponectin suppresses IkappaB kinase activation induced by tumor necrosis factor-alpha or high glucose in endothelial cells: role of cAMP and AMP kinase signaling.

American journal of physiology. Endocrinology and metabolism 20071201

Binding energy in the one-electron reductive cleavage of S-adenosylmethionine in lysine 2,3-aminomutase, a radical SAM enzyme.

Biochemistry 20071113

Iron-sulfur proteins as initiators of radical chemistry.

Natural product reports 20071001

Self-sacrifice in radical S-adenosylmethionine proteins.

Current opinion in chemical biology 20071001

Structural and signaling requirements of the human melanocortin 4 receptor for MAP kinase activation.

Regulatory peptides 20070816

5'-Peroxyadenosine and 5'-peroxyadenosylcobalamin as intermediates in the aerobic photolysis of adenosylcobalamin.

Biochemistry 20070619

Dioldehydrase: an essential role for potassium ion in the homolytic cleavage of the cobalt-carbon bond in adenosylcobalamin.

Biochemistry 20070619

Thiazole synthase from Escherichia coli: an investigation of the substrates and purified proteins required for activity in vitro.

The Journal of biological chemistry 20070615

Cyclic AMP signaling contributes to neural plasticity and hyperexcitability in AH sensory neurons following intestinal Trichinella spiralis-induced inflammation.

International journal for parasitology 20070601

Probing interactions from solvent-exchangeable protons and monovalent cations with the 1,2-propanediol-1-yl radical intermediate in the reaction of dioldehydrase.

Protein science : a publication of the Protein Society 20070601

S-Adenosylmethionine in protozoan parasites: functions, synthesis and regulation.

Molecular and biochemical parasitology 20070301

S-adenosylmethionine as an oxidant: the radical SAM superfamily.

Trends in biochemical sciences 20070301

Prostaglandin E(2) protects human lung fibroblasts from cigarette smoke extract-induced apoptosis via EP(2) receptor activation.

Journal of cellular physiology 20070101

Role of regulator of G-protein signaling 2 (RGS2) in periodontal ligament cells under mechanical stress.

Cell biochemistry and function 20070101

Phylogeny of the nematode genus Pristionchus and implications for biodiversity, biogeography and the evolution of hermaphroditism.

BMC evolutionary biology 20070101

Role of the [2Fe-2S]2+ cluster in biotin synthase: mutagenesis of the atypical metal ligand arginine 260.

Biochemistry 20061128

Enantiomeric free radicals and enzymatic control of stereochemistry in a radical mechanism: the case of lysine 2,3-aminomutases.

Biochemistry 20061024

Biotin synthase mechanism: mutagenesis of the YNHNLD conserved motif.

Biochemistry 20061010

Salbutamol inhibits trypsin-mediated production of CXCL8 by keratinocytes.

Cytokine 20061001

Characterization of an active spore photoproduct lyase, a DNA repair enzyme in the radical S-adenosylmethionine superfamily.

The Journal of biological chemistry 20060908

Quantum catalysis in B12-dependent methylmalonyl-CoA mutase: experimental and computational insights.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20060829

How an enzyme tames reactive intermediates: positioning of the active-site components of lysine 2,3-aminomutase during enzymatic turnover as determined by ENDOR spectroscopy.

Journal of the American Chemical Society 20060809

S-Adenosyl-L-methionine: beyond the universal methyl group donor.

Phytochemistry 20060801

Impaired complement-mediated phagocytosis by HIV type-1-infected human monocyte-derived macrophages involves a cAMP-dependent mechanism.

AIDS research and human retroviruses 20060701

The substrate radical of Escherichia coli oxygen-independent coproporphyrinogen III oxidase HemN.

The Journal of biological chemistry 20060609

Phosphorylation of paracellin-1 at Ser217 by protein kinase A is essential for localization in tight junctions.

Journal of cell science 20060501

Insights into the hydrogen-abstraction reactions of diol dehydratase: relevance to the catalytic mechanism and suicide inactivation.

Journal of the American Chemical Society 20060315

Cofactor dependence of reduction potentials for [4Fe-4S]2+/1+ in lysine 2,3-aminomutase.

Biochemistry 20060314

Signaling pathways for modulation of mouse sperm motility by adenosine and catecholamine agonists.

Biology of reproduction 20060301

Multiple signalling pathways involved in beta2-adrenoceptor-mediated glucose uptake in rat skeletal muscle cells.

British journal of pharmacology 20060201

Computational insights into the mechanism of radical generation in B12-dependent methylmalonyl-CoA mutase.

Journal of the American Chemical Society 20060201

Cyclooxygenase-2 induction by bradykinin in aortic vascular smooth muscle cells.

American journal of physiology. Heart and circulatory physiology 20060101

Adenylate cyclase 5 and KCa1.1 channel are required for EGFR up-regulation of PCNA in native contractile rat basilar artery smooth muscle.

The Journal of physiology 20060101

Vitamin B12 and methionine synthesis: a critical review. Is nature's most beautiful cofactor misunderstood?

BioFactors (Oxford, England) 20060101

Preliminary mechanistic studies on the smooth muscle relaxant effect of hydroalcoholic extract of Curcuma caesia.

Journal of herbal pharmacotherapy 20060101

Stabilisation of methylene radicals by cob(II)alamin in coenzyme B12 dependent mutases.

Chemistry (Weinheim an der Bergstrasse, Germany) 20051223

Relaxant effect of adrenomedullin on bovine isolated iris sphincter muscle under resting conditions.

Clinical and experimental pharmacology & physiology 20050801

Divergent mechanisms of suicide inactivation for ethanolamine ammonia-lyase.

Journal of the American Chemical Society 20050622

A nucleosidase required for in vivo function of the S-adenosyl-L-methionine radical enzyme, biotin synthase.

Chemistry & biology 20050501

Nitric oxide inhibits mammalian methylmalonyl-CoA mutase.

The Journal of biological chemistry 20050318

Isotope effects for deuterium transfer between substrate and coenzyme in adenosylcobalamin-dependent glutamate mutase.

Biochemistry 20050222

Spectroscopic approaches to elucidating novel iron-sulfur chemistry in the 'radical-Sam' protein superfamily.

Inorganic chemistry 20050221

Pyruvate formate-lyase activating enzyme: elucidation of a novel mechanism for glycyl radical formation.

Archives of biochemistry and biophysics 20050101

Natural history of S-adenosylmethionine-binding proteins.

BMC structural biology 20050101

Phosphoramidate derivatives of 2',5'-dideoxyadenosine as potential inhibitors of the EDHF phenomenon.

Nucleosides, nucleotides & nucleic acids 20050101

Further investigation on the turnover of Escherichia coli biotin synthase with dethiobiotin and 9-mercaptodethiobiotin as substrates.

Biochemistry 20041228

Nicotine and epibatidine triggered prolonged rise in calcium and TH gene transcription in PC12 cells.

European journal of pharmacology 20041203

Insight into the polar reactivity of the onium chalcogen analogues of S-adenosyl-L-methionine.

Biochemistry 20041026

Suicide inactivation of dioldehydratase by glycolaldehyde and chloroacetaldehyde: an examination of the reaction mechanism.

Journal of the American Chemical Society 20041006

S-adenosylmethionine radical enzymes.

Bioorganic chemistry 20041001

Structure and function of radical SAM enzymes.

Current opinion in chemical biology 20041001

Escherichia coli lipoyl synthase binds two distinct [4Fe-4S] clusters per polypeptide.

Biochemistry 20040921

Partial B-to-A DNA transition upon minor groove binding of protein Sac7d monitored by Raman spectroscopy.

Biochemistry 20040803

When a spectator turns killer: suicidal electron transfer from cobalamin in methylmalonyl-CoA mutase.

Biochemistry 20040706

Computational investigation of hydrogen abstraction from 2-aminoethanol by the 1,5-dideoxyribose-5-yl radical: a model study of a reaction occurring in the active site of ethanolamine ammonia lyase.

Chemistry (Weinheim an der Bergstrasse, Germany) 20040607

Lipoyl synthase requires two equivalents of S-adenosyl-L-methionine to synthesize one equivalent of lipoic acid.

Biochemistry 20040601

S-adenosylmethionine: nothing goes to waste.

Trends in biochemical sciences 20040501

Post-translational formylglycine modification of bacterial sulfatases by the radical S-adenosylmethionine protein AtsB.

The Journal of biological chemistry 20040409

Pre-steady-state measurement of intrinsic secondary tritium isotope effects associated with the homolysis of adenosylcobalamin and the formation of 5'-deoxyadensosine in glutamate mutase.

Biochemistry 20040302

Role of the [2Fe-2S] cluster in recombinant Escherichia coli biotin synthase.

Biochemistry 20040224

Activation of alpha1A-adrenergic receptor promotes differentiation of rat-1 fibroblasts to a smooth muscle-like phenotype.

BMC cell biology 20040101

Crystal structure of coproporphyrinogen III oxidase reveals cofactor geometry of Radical SAM enzymes.

The EMBO journal 20031201

Evidence for multiple distinctly localized adenylyl cyclase isoforms in mammalian spermatozoa.

Molecular reproduction and development 20031001

Coordination and mechanism of reversible cleavage of S-adenosylmethionine by the [4Fe-4S] center in lysine 2,3-aminomutase.

Journal of the American Chemical Society 20031001

The first experimental test of the hypothesis that enzymes have evolved to enhance hydrogen tunneling.

Journal of the American Chemical Society 20030910

Decreased adenylyl cyclase and cAMP-dependent protein kinase activities inhibit the cytotoxic function of human natural killer cells.

Human immunology 20030901

Novel mutations in a Thai patient with methylmalonic acidemia.

Molecular genetics and metabolism 20030801

Structural studies of the interaction of S-adenosylmethionine with the [4Fe-4S] clusters in biotin synthase and pyruvate formate-lyase activating enzyme.

Protein science : a publication of the Protein Society 20030701

HU protein employs similar mechanisms of minor-groove recognition in binding to different B-DNA sites: demonstration by Raman spectroscopy.

Biochemistry 20030624

Epimerization at carbon-5' of (5'R)-[5'-2H]adenosylcobalamin by ribonucleoside triphosphate reductase: cysteine 408-independent cleavage of the Co-C5' bond.

Biochemistry 20030422

The generation of 5'-deoxyadenosyl radicals by adenosylmethionine-dependent radical enzymes.

Current opinion in chemical biology 20030401

Control of adenosylmethionine-dependent radical generation in biotin synthase: a kinetic and thermodynamic analysis of substrate binding to active and inactive forms of BioB.

Biochemistry 20030311

An automatic method to generate force-field parameters for hetero-compounds.

Acta crystallographica. Section D, Biological crystallography 20030201

Internal degrees of freedom, structural motifs, and conformational energetics of the 5'-deoxyadenosyl radical: implications for function in adenosylcobalamin-dependent enzymes. A computational study.

Journal of the American Chemical Society 20030115

The PLP-dependent biotin synthase from Escherichia coli: mechanistic studies.

FEBS letters 20021218

An anchoring role for FeS clusters: chelation of the amino acid moiety of S-adenosylmethionine to the unique iron site of the [4Fe-4S] cluster of pyruvate formate-lyase activating enzyme.

Journal of the American Chemical Society 20020925

Roles for both cyclic GMP and cyclic AMP in the inhibition of collagen-induced platelet aggregation by nitroprusside.

British journal of haematology 20020601

Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli.

The Journal of biological chemistry 20020419

Direct H atom abstraction from spore photoproduct C-6 initiates DNA repair in the reaction catalyzed by spore photoproduct lyase: evidence for a reversibly generated adenosyl radical intermediate.

Journal of the American Chemical Society 20020327

Electron-nuclear double resonance spectroscopic evidence that S-adenosylmethionine binds in contact with the catalytically active [4Fe-4S](+) cluster of pyruvate formate-lyase activating enzyme.

Journal of the American Chemical Society 20020327

Cyclic AMP mediated GSTP1 gene activation in tumor cells involves the interaction of activated CREB-1 with the GSTP1 CRE: a novel mechanism of cellular GSTP1 gene regulation.

Journal of cellular biochemistry 20020101

Adenosylmethionine as a source of 5'-deoxyadenosyl radicals.

Current opinion in chemical biology 20011001

Enzymology of carbon-sulfur bond formation.

Current opinion in chemical biology 20011001

Interaction of the substrate radical and the 5'-deoxyadenosine-5'-methyl group in vitamin B(12) coenzyme-dependent ethanolamine deaminase.

Journal of the American Chemical Society 20010905

Characterization of an allylic analogue of the 5'-deoxyadenosyl radical: an intermediate in the reaction of lysine 2,3-aminomutase.

Biochemistry 20010703

Reactivity of [Fe4S4(SR)4]2-,3- clusters with sulfonium cations: analogue reaction systems for the initial step in biotin synthase catalysis.

Inorganic chemistry 20010604

Electron transfer in the substrate-dependent suicide inactivation of lysine 5,6-aminomutase.

Biochemistry 20010501

Activation of class III ribonucleotide reductase by flavodoxin: a protein radical-driven electron transfer to the iron-sulfur center.

Biochemistry 20010327

cAMP-independent phosphorylation activation of CFTR by G proteins in native human sweat duct.

American journal of physiology. Cell physiology 20010301

Identification of lysine 346 as a functionally important residue for pyridoxal 5'-phosphate binding and catalysis in lysine 2, 3-aminomutase from Bacillus subtilis.

Biochemistry 20010116

5'-Deoxyadenosine contacts the substrate radical intermediate in the active site of ethanolamine ammonia-lyase: 2H and 13C electron nuclear double resonance studies.

Biochemistry 20010109

Biosynthesis of biotin and lipoic acid.

Vitamins and hormones 20010101

Radical mechanisms of enzymatic catalysis.

Annual review of biochemistry 20010101

The role of radicals in enzymatic processes.

Chemical record (New York, N.Y.) 20010101

Conversion of 2',3'-dideoxyadenosine (ddA) and 2',3'-didehydro-2',3'-dideoxyadenosine (d4A) to their corresponding aryloxyphosphoramidate derivatives markedly potentiates their activity against human immunodeficiency virus and hepatitis B virus.

FEBS letters 19970630

Sensitization of C-fibres by prostaglandin E2 in the rat is inhibited by guanosine 5'-O-(2-thiodiphosphate), 2',5'-dideoxyadenosine and Walsh inhibitor peptide.

Neuroscience 19960301